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Wolbachia Workshops


 Discover the Microbes Within: The Wolbachia Project

Professional Development Workshop - The Marine Biological Laboratory, Woods Hole, MA





Discover the Microbes Within!
Teacher Enhancement Workshop on Symbiosis

 The Marine Biological Laboratory

Cosponsored by the NASA Astrobiology Institute and the National Science Foundation (FIBR: Integrative Studies of Wolbachia-Eukaryotic Interactions; Genomes to
Communities and Back NSF 0328363).

Coorganized by Dr. Seth Bordenstein, Michele Bahr, and Dr. Jack Werren

This workshop is designed for high school and undergraduate educators in the biological sciences in order to enhance content background, provide teaching tools, and bring new life to science classes. Approximately 25 selected applicants will learn about the diverse ways that bacteria evolve and symbiotically interact with insects - in an environment that fosters close interactions with leading research scientists and other teachers. Basic topics and lesson plans cover insect collection, insect biodiversity, the mutualistic and parasitic bacteria (Wolbachia) that live within them, DNA extraction, and simple molecular biology and evolutionary analysis skills. The workshop emphasizes activities and experimental systems that can be used easily in the classroom to teach basic biological principles.
Workshop 2006 - March 10-12

Friday, March 10

8 a.m. Breakfast, Swope Dining Room


9 a.m. Course Welcome and Introductions - Michele Bahr


9:30 a.m. “Bacterial Endosymbionts of Insects” Lecture (! ppt file 40 MB!) – Jack Werren


10:30 a.m. Lab safety - Michele Bahr


Noon Lunch, Swope Dining Room


1 p.m. Insect Identification and Curation Laboratory: Lecture (! ppt file 16MB!)  - Lab Exercise (word file) - Bob Minckley


6 p.m. Dinner, Swope Dining Room


7 p.m. (Brief lecture w/ Coffee, Tea, and Dessert) “Impacts of DNA-based technologies and PCR basics” (! ppt file 5MB!) – Lecture with Seth Bordenstein (Starr 209)

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Saturday, March 11

8 a.m. Breakfast, Swope Dining Room


9 a.m. Genomic DNA Extraction Laboratory (! ppt file 100KB!) - George Wolfe


11 a.m. PCR Laboratory (word file) - George Wolfe


Noon Lunch, Swope Dining Room


2:30 p.m. “Principles of Gel Electrophoresis” Lecture (! ppt file 10MB!) and Gel Electrophoresis Laboratory (word file)- Michael Clark


5:30 p.m. Wine, Beer, and Soft Drinks, Ebert Hall Lobby


6 p.m. Dinner, Swope Dining Room


7 p.m. “Sequencing PCR Products and Bioinformatic Tools” Lecture - Jennifer Wernegreen

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Sunday, March 12

8 a.m. Breakfast, Swope Dining Room


9 a.m. Bioinformatics -Lecture (! ppt file 1.5MB!)  - Laboratory Excercise (word file) - Seth Bordenstein


10 a.m DNA Sequence Alignments and Phylogenetic Trees - Lecture (! ppt file 1MB!) - Laboratory Exercise (word file) – Laura Baldo


11 a.m. Review, Q & A, hand in course evaluations, lab cleanup


12 p.m. Teacher departure


Afternoon Faculty departure


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Workshop 2005 - March 18-20

Happy workshopers!

Friday, March 18

10 a.m. Course Welcome and Introductions - Michele Bahr


10:30 a.m. “Bacterial Endosymbionts of Insects” Lecture – Jack Werren


11:30 a.m. Lab safety - Michele Bahr


Noon Lunch, Swope Dining Room


1:15 p.m. Insect Identification and Curation: Lecture (!ppt file 22.2 MB!) - Lab Exercise (word file) - Bob Minckley


5:30 p.m. Dinner, Swope Dining Room


7 p.m. (Brief lecture w/ Drinks and Munchies) “How Molecular Genetic Techniques have Revolutionized Microbiology and Introduction to PCR” (!ppt file 6 MB!) – Lecture with Seth Bordenstein

Saturday, March 19

8 a.m. Breakfast, Swope Dining Room


9 a.m. Genomic DNA Extraction Laboratory (! ppt file 113 KB !) - George Wolfe


Noon Lunch, Swope Dining Room


1:15 p.m. PCR Laboratory (word file) - George Wolfe


5:30 p.m. Dinner, Swope Dining Room


7 p.m. “Principles of Gel Electrophoresis” (!! ppt file 63 MB!!) Lecture and Gel Electrophoresis Laboratory (word file) - Michael Clark


8 p.m. “Sequencing PCR Products and Bioinformatic Tools” Lecture (while gels are running) - Jennifer Wernegreen

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Sunday, March 20

8 a.m. Breakfast, Swope Dining Room


9 a.m. Bioinformatics: Lecture (! ppt file 336 KB!) and Laboratory Exercise (word file) - Seth Bordenstein


10:30 a.m. Alignments and Phylogenies Lecture (! ppt file 63 KB!) – Laura Baldo


11 a.m. Review, Q & A, course evaluation, lab cleanup


Noon Teacher departure


Afternoon Faculty departure

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Please send any comments or questions to dolivei2@mail.rochester.edu
© 2006 by The University of Rochester. All Rights Reserved.
This document last modified 2008-03-26